Transfer RNA genes of Drosophila melanogaster
نویسندگان
چکیده
Three recombinant plasmids containing randomly sheared genomic D. melanogaster tRNAs have been identified and characterized in detail. One of these, the plasmid 14C4, has a D. melanogaster (Dm) DNA segment of 18 kb, and has three tRNA2Arg and two tRNAAsN genes. The second plasmid, 38B10, has tRNAHis genes, while the third plasmid, 63H5, contains coding sequences for tRNA2Asp. The Dm DNA segments in each recombinant plasmid are derived from unique cytogenetic loci. 14C4 is from 84 F, 38B10 is from 48 F and 63H5 is from 70 A.
منابع مشابه
Aconitase and Developmental EndPointsasEarly IndicatorsofCellularToxicity Induced by Xenobiotics in Drosophila Melanogaster
Background: In this study, the toxicity of the different xenobiotics was tested on the fruit fly Drosophila melanogaster model system. Methods: Fly larvae were raised on food supplemented with xenobioticsat different concentrations (sodium nitroprusside (0.1-1.5 mM), S-nitrosoglutathione (0.5-4 mM), and potassium ferrocyanide (1 mM)). Emergence of flies, food intake by larvae, and pupation h...
متن کاملConcentration dependent effect of morphine, aspirin, capsaicin and chili pepper hydro alcoholic extract on thermal and chemical pain model in fruit fly (Drosophila melanogaster)
Introduction: Pain research using animal models is related to ethical concerns, so invertebrates and insects have been recommended by researchers. In the present study, the nociceptive and antinociceptive effects of capsaicin, aspirin, morphine and chili extract were examined using fruit fly (Drosophila melanogaster) as an alternative for rodent pain model. Methods: Stage 3 of larvae and ad...
متن کاملToxicological Evaluation of a New Lepidopteran Insecticide, Flubendiamide, in Non-Target Drosophila melanogaster Meigen (Diptera: Drosophilidae)
Background: Flubendiamide, comparatively a new pesticide designed to eradicate lepidopteran insect pests is known to have low risk to birds, mammals, fish, algae, honey bees, non-target arthropods, earthworms, soil macro- and micro-organisms, non-target plants as well as sewage treatment organisms; however, the risk assessment for aquatic invertebrates from metabolite could not be finalized wit...
متن کاملIn Vivo RNAi Rescue in Drosophila melanogaster with Genomic Transgenes from Drosophila pseudoobscura
BACKGROUND Systematic, large-scale RNA interference (RNAi) approaches are very valuable to systematically investigate biological processes in cell culture or in tissues of organisms such as Drosophila. A notorious pitfall of all RNAi technologies are potential false positives caused by unspecific knock-down of genes other than the intended target gene. The ultimate proof for RNAi specificity is...
متن کاملLocalization of tRNA genes in the salivary chromosomes of Drosophila by RNA:DNA hybridization.
HE methods for in situ molecular hybridization to nuclei and chromosomes Thave been developed due primarily to the efforts of GALL and PARDUE (1969), PARDUE et al. (1970) and JOHN, BIRNSTIEL and JONES (1969). In theory the procedure permits the use of any fraction of labeled RNA or DNA to locate the site of specific binding in a genome, provided there is enough radioactivity for detection by au...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Nucleic acids research
دوره 8 13 شماره
صفحات -
تاریخ انتشار 1980